Part:BBa_K538302:Design

pBAD + RBS + SheDnaK

pBAD:SDnaK K538302

This part is one of team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011]'s cold resistance BioBricks, also known as a CryoBrick. When transformed into E. coli, it should bestow a certain degree of cold resistance. It was placed under control of the strong arabinose-induced promoter derived from pBAD by team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] (BBa_K206000). This means it will only be active in presence of arabinose, effectively turning the Brick off by default, and allowing it to be switched on by adding arabinose. When the arabinose is consumed, the Brick reverts to its off state. The promoter was taken from 2011's Spring Distribution Kit, as was the incorporated RBS. The coding region of this Brick is a new submission to the registry, also submitted separately as BBa_K538002.

For more information about the subparts' purpose and design, refer to their main page or design page, respectively.

• BBa_K206000
  • Main Page
  • Design Page
• BBa_B0034
  • Main Page
  • Design Page
• BBa_K538002
  • Main Page
  • Design Page

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 903
  Illegal BamHI site found at 65
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]